HF2: a double-stranded DNA tailed haloarchaeal virus with a mosaic genome
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The double-stranded DNA of cauliflower mosaic virus.
Examination of the physical structure of cauliflower mosaic virus DNA by electron microscopy and ultracentrifugal analysis showed that the DNA could be isolated mainly as a circular molecule of mol. wt. 4"7 x Io 6. Nearest-neighbour frequency analysis showed that the DNA has a chemical structure which exhibits close similarities to that of the host cauliflower DNA. The relationships of this pla...
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Two crucial steps in the virus life cycle are genome encapsidation to form an infective virion and genome exit to infect the next host cell. In most icosahedral double-stranded (ds) DNA viruses, the viral genome enters and exits the capsid through a unique vertex. Internal membrane-containing viruses possess additional complexity as the genome must be translocated through the viral membrane bil...
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Folding mechanisms of proteins incorporated within supramolecular assemblies, including viruses, are little understood and may differ fundamentally from folding mechanisms of small globular proteins. We describe a novel Raman dynamic probe of hydrogen-isotope exchange to investigate directly these protein folding/assembly pathways. The method is applied to subunit folding in assembly intermedia...
متن کاملA double-stranded DNA rotaxane.
Mechanically interlocked molecules such as rotaxanes and catenanes have potential as components of molecular machinery. Rotaxanes consist of a dumb-bell-shaped molecule encircled by a macrocycle that can move unhindered along the axle, trapped by bulky stoppers. Previously, rotaxanes have been made from a variety of molecules, but not from DNA. Here, we report the design, assembly and character...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2002
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2002.02890.x